Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Identifieur interne : 000226 ( Ncbi/Merge ); précédent : 000225; suivant : 000227Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Auteurs : D S Hwang [États-Unis] ; A. KornbergSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), ADN bactérien (isolement et purification), Cartographie de restriction, Cinétique, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Facteurs d'intégration de l'hôte, Gènes bactériens, Liaison aux protéines, Oligodésoxyribonucléotides, Plasmides, Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN, Séquence nucléotidique.
- MESH :
- génétique : ADN bactérien, Escherichia coli.
- isolement et purification : ADN bactérien.
- métabolisme : Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- Cartographie de restriction, Cinétique, Données de séquences moléculaires, Facteurs d'intégration de l'hôte, Gènes bactériens, Liaison aux protéines, Oligodésoxyribonucléotides, Plasmides, Réplication de l'ADN, Séquence nucléotidique.
English descriptors
- KwdEn :
- Bacterial Proteins (metabolism), Base Sequence, DNA Replication, DNA, Bacterial (genetics), DNA, Bacterial (isolation & purification), DNA-Binding Proteins (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Genes, Bacterial, Integration Host Factors, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Plasmids, Protein Binding, Restriction Mapping.
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , isolation & purification : DNA, Bacterial.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- Base Sequence, DNA Replication, Genes, Bacterial, Integration Host Factors, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Plasmids, Protein Binding, Restriction Mapping.
Abstract
Opening of the three tandem repeats of a 13-mer in the replication origin (oriC) of Escherichia coli is a prime event in the replication in vitro of minichromosomes (Bramhill, D., and Kornberg, A. (1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical density in the plasmid to open this region. The extent of opening, as judged by cleavage by a single-strand-specific endonuclease (i.e. P1 nuclease), correlated closely with replication of the oriC plasmid. In an initial complex, preceding opening of the 13-mers, the footprint of DnaA protein bound by ATP covered its four 9-mer recognition sequences. The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific.
PubMed: 1429655
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(1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical density in the plasmid to open this region. The extent of opening, as judged by cleavage by a single-strand-specific endonuclease (i.e. P1 nuclease), correlated closely with replication of the oriC plasmid. In an initial complex, preceding opening of the 13-mers, the footprint of DnaA protein bound by ATP covered its four 9-mer recognition sequences. The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1429655</PMID><DateCompleted><Year>1992</Year><Month>12</Month><Day>16</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9258</ISSN><JournalIssue CitedMedium="Print"><Volume>267</Volume><Issue>32</Issue><PubDate><Year>1992</Year><Month>Nov</Month><Day>15</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J. Biol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.</ArticleTitle><Pagination><MedlinePgn>23083-6</MedlinePgn></Pagination><Abstract><AbstractText>Opening of the three tandem repeats of a 13-mer in the replication origin (oriC) of Escherichia coli is a prime event in the replication in vitro of minichromosomes (Bramhill, D., and Kornberg, A. (1988) Cell 54, 915-918). DnaA, the initiator protein, requires protein HU or IHF, along with a millimolar level of ATP and negative superhelical density in the plasmid to open this region. The extent of opening, as judged by cleavage by a single-strand-specific endonuclease (i.e. P1 nuclease), correlated closely with replication of the oriC plasmid. In an initial complex, preceding opening of the 13-mers, the footprint of DnaA protein bound by ATP covered its four 9-mer recognition sequences. The footprint of the nucleotide-free form of the protein, by contrast, was more extensive and thus, less specific.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>D S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kornberg</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Biol Chem</MedlineTA><NlmUniqueID>2985121R</NlmUniqueID><ISSNLinking>0021-9258</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C066312">DnaA protein, Bacteria</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034982">Integration Host Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C040330">histone-like protein HU, bacteria</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004261" MajorTopicYN="Y">DNA Replication</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="N">Genes, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D034982" MajorTopicYN="N">Integration Host Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>11</Month><Day>15</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>11</Month><Day>15</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1429655</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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